1× immuno wash Search Results


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horseradish peroxidase conjugated goat antimouse igg antibody  (Jackson Immuno)
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cy5 conjugated donkey anti rabbit secondary antibody  (Jackson Immuno)
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Fig. 2 Monosynaptic rabies viral tracing reveals RSC intrinsic inputs to M2-projecting and AD-projecting RSCg neurons in C57BL/6 mice. A Schematic of monosynaptic retrograde rabies tracing (RV) of synaptic inputs to M2-projecting RSCg cells. The rAAV2-retro-Cre injection in M2 retrogradely drives Cre expression in M2-projecting RSC; then the Cre dependent AAV helper virus (AAV8-hSyn-DIO-TC66T-2A-EGFP-2A- OG) is injected into RSCg, followed by injection of an EnvA-pseudotyped SAD ΔG rabies virus three weeks later. B Immunostaining and microscopy images depict Cre positive neurons in the M2 injection site. rAAV2-retro-Cre labeled neurons are stained by a <t>Cy5-conjugated</t> secondary antibody and visualized as pink in the enlarged inset. C, D Coronal sections of injection in RSCg. Injection sites are shown in white boxes (C) with magnified images in (D). EGFP-labeled AAV-helper virus expressed in M2-projecting Cre+ neurons. Rabies is labeled by DsRed, DAPI is blue. White arrowheads indicate EGFP and DsRed double-labeled starter neurons. E Quantitative mapping of the connection strength of the intrinsic RSC subregion inputs to M2-projetcing RSCg neurons. Connection strength index (CSI) is calculated by the number of presynaptic input neurons divided by the number of starter neurons in RSCg. The individual circle represents one case (n = 5). Data are measured from M2-projecting RSCg neurons (N = 5 cases) and AD-projecting RSCg neurons (N = 5 cases) and are presented as the mean ± s.e.m. F, G Example of input- mapped local RSC subregions along the longitudinal axis. AP number indicates the distance relative to bregma. G represents magnified views corresponding to the panels in (F), indicating that the intrinsic connections vary across different layers along the anterior/posterior axis. H–L follow the same order as (A)–(E) and reveal RSC subregions inputs to AD-projecting RSCg neurons.
Cy5 Conjugated Donkey Anti Rabbit Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti mouse igg hrp antibody  (Jackson Immuno)
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Jackson Immuno goat anti mouse igg hrp antibody
Fig. 2 Monosynaptic rabies viral tracing reveals RSC intrinsic inputs to M2-projecting and AD-projecting RSCg neurons in C57BL/6 mice. A Schematic of monosynaptic retrograde rabies tracing (RV) of synaptic inputs to M2-projecting RSCg cells. The rAAV2-retro-Cre injection in M2 retrogradely drives Cre expression in M2-projecting RSC; then the Cre dependent AAV helper virus (AAV8-hSyn-DIO-TC66T-2A-EGFP-2A- OG) is injected into RSCg, followed by injection of an EnvA-pseudotyped SAD ΔG rabies virus three weeks later. B Immunostaining and microscopy images depict Cre positive neurons in the M2 injection site. rAAV2-retro-Cre labeled neurons are stained by a <t>Cy5-conjugated</t> secondary antibody and visualized as pink in the enlarged inset. C, D Coronal sections of injection in RSCg. Injection sites are shown in white boxes (C) with magnified images in (D). EGFP-labeled AAV-helper virus expressed in M2-projecting Cre+ neurons. Rabies is labeled by DsRed, DAPI is blue. White arrowheads indicate EGFP and DsRed double-labeled starter neurons. E Quantitative mapping of the connection strength of the intrinsic RSC subregion inputs to M2-projetcing RSCg neurons. Connection strength index (CSI) is calculated by the number of presynaptic input neurons divided by the number of starter neurons in RSCg. The individual circle represents one case (n = 5). Data are measured from M2-projecting RSCg neurons (N = 5 cases) and AD-projecting RSCg neurons (N = 5 cases) and are presented as the mean ± s.e.m. F, G Example of input- mapped local RSC subregions along the longitudinal axis. AP number indicates the distance relative to bregma. G represents magnified views corresponding to the panels in (F), indicating that the intrinsic connections vary across different layers along the anterior/posterior axis. H–L follow the same order as (A)–(E) and reveal RSC subregions inputs to AD-projecting RSCg neurons.
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horseradish peroxidase conjugated goat anti mouse secondary antibody  (Bio-Rad)
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Bio-Rad horseradish peroxidase conjugated goat anti mouse secondary antibody
Fig. 2 Monosynaptic rabies viral tracing reveals RSC intrinsic inputs to M2-projecting and AD-projecting RSCg neurons in C57BL/6 mice. A Schematic of monosynaptic retrograde rabies tracing (RV) of synaptic inputs to M2-projecting RSCg cells. The rAAV2-retro-Cre injection in M2 retrogradely drives Cre expression in M2-projecting RSC; then the Cre dependent AAV helper virus (AAV8-hSyn-DIO-TC66T-2A-EGFP-2A- OG) is injected into RSCg, followed by injection of an EnvA-pseudotyped SAD ΔG rabies virus three weeks later. B Immunostaining and microscopy images depict Cre positive neurons in the M2 injection site. rAAV2-retro-Cre labeled neurons are stained by a <t>Cy5-conjugated</t> secondary antibody and visualized as pink in the enlarged inset. C, D Coronal sections of injection in RSCg. Injection sites are shown in white boxes (C) with magnified images in (D). EGFP-labeled AAV-helper virus expressed in M2-projecting Cre+ neurons. Rabies is labeled by DsRed, DAPI is blue. White arrowheads indicate EGFP and DsRed double-labeled starter neurons. E Quantitative mapping of the connection strength of the intrinsic RSC subregion inputs to M2-projetcing RSCg neurons. Connection strength index (CSI) is calculated by the number of presynaptic input neurons divided by the number of starter neurons in RSCg. The individual circle represents one case (n = 5). Data are measured from M2-projecting RSCg neurons (N = 5 cases) and AD-projecting RSCg neurons (N = 5 cases) and are presented as the mean ± s.e.m. F, G Example of input- mapped local RSC subregions along the longitudinal axis. AP number indicates the distance relative to bregma. G represents magnified views corresponding to the panels in (F), indicating that the intrinsic connections vary across different layers along the anterior/posterior axis. H–L follow the same order as (A)–(E) and reveal RSC subregions inputs to AD-projecting RSCg neurons.
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donkey anti rabbit cy3 fluophore  (Jackson Immuno)
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Jackson Immuno donkey anti rabbit cy3 fluophore
Fig. 2 Monosynaptic rabies viral tracing reveals RSC intrinsic inputs to M2-projecting and AD-projecting RSCg neurons in C57BL/6 mice. A Schematic of monosynaptic retrograde rabies tracing (RV) of synaptic inputs to M2-projecting RSCg cells. The rAAV2-retro-Cre injection in M2 retrogradely drives Cre expression in M2-projecting RSC; then the Cre dependent AAV helper virus (AAV8-hSyn-DIO-TC66T-2A-EGFP-2A- OG) is injected into RSCg, followed by injection of an EnvA-pseudotyped SAD ΔG rabies virus three weeks later. B Immunostaining and microscopy images depict Cre positive neurons in the M2 injection site. rAAV2-retro-Cre labeled neurons are stained by a <t>Cy5-conjugated</t> secondary antibody and visualized as pink in the enlarged inset. C, D Coronal sections of injection in RSCg. Injection sites are shown in white boxes (C) with magnified images in (D). EGFP-labeled AAV-helper virus expressed in M2-projecting Cre+ neurons. Rabies is labeled by DsRed, DAPI is blue. White arrowheads indicate EGFP and DsRed double-labeled starter neurons. E Quantitative mapping of the connection strength of the intrinsic RSC subregion inputs to M2-projetcing RSCg neurons. Connection strength index (CSI) is calculated by the number of presynaptic input neurons divided by the number of starter neurons in RSCg. The individual circle represents one case (n = 5). Data are measured from M2-projecting RSCg neurons (N = 5 cases) and AD-projecting RSCg neurons (N = 5 cases) and are presented as the mean ± s.e.m. F, G Example of input- mapped local RSC subregions along the longitudinal axis. AP number indicates the distance relative to bregma. G represents magnified views corresponding to the panels in (F), indicating that the intrinsic connections vary across different layers along the anterior/posterior axis. H–L follow the same order as (A)–(E) and reveal RSC subregions inputs to AD-projecting RSCg neurons.
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Fig. 2 Monosynaptic rabies viral tracing reveals RSC intrinsic inputs to M2-projecting and AD-projecting RSCg neurons in C57BL/6 mice. A Schematic of monosynaptic retrograde rabies tracing (RV) of synaptic inputs to M2-projecting RSCg cells. The rAAV2-retro-Cre injection in M2 retrogradely drives Cre expression in M2-projecting RSC; then the Cre dependent AAV helper virus (AAV8-hSyn-DIO-TC66T-2A-EGFP-2A- OG) is injected into RSCg, followed by injection of an EnvA-pseudotyped SAD ΔG rabies virus three weeks later. B Immunostaining and microscopy images depict Cre positive neurons in the M2 injection site. rAAV2-retro-Cre labeled neurons are stained by a <t>Cy5-conjugated</t> secondary antibody and visualized as pink in the enlarged inset. C, D Coronal sections of injection in RSCg. Injection sites are shown in white boxes (C) with magnified images in (D). EGFP-labeled AAV-helper virus expressed in M2-projecting Cre+ neurons. Rabies is labeled by DsRed, DAPI is blue. White arrowheads indicate EGFP and DsRed double-labeled starter neurons. E Quantitative mapping of the connection strength of the intrinsic RSC subregion inputs to M2-projetcing RSCg neurons. Connection strength index (CSI) is calculated by the number of presynaptic input neurons divided by the number of starter neurons in RSCg. The individual circle represents one case (n = 5). Data are measured from M2-projecting RSCg neurons (N = 5 cases) and AD-projecting RSCg neurons (N = 5 cases) and are presented as the mean ± s.e.m. F, G Example of input- mapped local RSC subregions along the longitudinal axis. AP number indicates the distance relative to bregma. G represents magnified views corresponding to the panels in (F), indicating that the intrinsic connections vary across different layers along the anterior/posterior axis. H–L follow the same order as (A)–(E) and reveal RSC subregions inputs to AD-projecting RSCg neurons.
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Fig. 2 Monosynaptic rabies viral tracing reveals RSC intrinsic inputs to M2-projecting and AD-projecting RSCg neurons in C57BL/6 mice. A Schematic of monosynaptic retrograde rabies tracing (RV) of synaptic inputs to M2-projecting RSCg cells. The rAAV2-retro-Cre injection in M2 retrogradely drives Cre expression in M2-projecting RSC; then the Cre dependent AAV helper virus (AAV8-hSyn-DIO-TC66T-2A-EGFP-2A- OG) is injected into RSCg, followed by injection of an EnvA-pseudotyped SAD ΔG rabies virus three weeks later. B Immunostaining and microscopy images depict Cre positive neurons in the M2 injection site. rAAV2-retro-Cre labeled neurons are stained by a <t>Cy5-conjugated</t> secondary antibody and visualized as pink in the enlarged inset. C, D Coronal sections of injection in RSCg. Injection sites are shown in white boxes (C) with magnified images in (D). EGFP-labeled AAV-helper virus expressed in M2-projecting Cre+ neurons. Rabies is labeled by DsRed, DAPI is blue. White arrowheads indicate EGFP and DsRed double-labeled starter neurons. E Quantitative mapping of the connection strength of the intrinsic RSC subregion inputs to M2-projetcing RSCg neurons. Connection strength index (CSI) is calculated by the number of presynaptic input neurons divided by the number of starter neurons in RSCg. The individual circle represents one case (n = 5). Data are measured from M2-projecting RSCg neurons (N = 5 cases) and AD-projecting RSCg neurons (N = 5 cases) and are presented as the mean ± s.e.m. F, G Example of input- mapped local RSC subregions along the longitudinal axis. AP number indicates the distance relative to bregma. G represents magnified views corresponding to the panels in (F), indicating that the intrinsic connections vary across different layers along the anterior/posterior axis. H–L follow the same order as (A)–(E) and reveal RSC subregions inputs to AD-projecting RSCg neurons.
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Image Search Results


Fig. 2 Monosynaptic rabies viral tracing reveals RSC intrinsic inputs to M2-projecting and AD-projecting RSCg neurons in C57BL/6 mice. A Schematic of monosynaptic retrograde rabies tracing (RV) of synaptic inputs to M2-projecting RSCg cells. The rAAV2-retro-Cre injection in M2 retrogradely drives Cre expression in M2-projecting RSC; then the Cre dependent AAV helper virus (AAV8-hSyn-DIO-TC66T-2A-EGFP-2A- OG) is injected into RSCg, followed by injection of an EnvA-pseudotyped SAD ΔG rabies virus three weeks later. B Immunostaining and microscopy images depict Cre positive neurons in the M2 injection site. rAAV2-retro-Cre labeled neurons are stained by a Cy5-conjugated secondary antibody and visualized as pink in the enlarged inset. C, D Coronal sections of injection in RSCg. Injection sites are shown in white boxes (C) with magnified images in (D). EGFP-labeled AAV-helper virus expressed in M2-projecting Cre+ neurons. Rabies is labeled by DsRed, DAPI is blue. White arrowheads indicate EGFP and DsRed double-labeled starter neurons. E Quantitative mapping of the connection strength of the intrinsic RSC subregion inputs to M2-projetcing RSCg neurons. Connection strength index (CSI) is calculated by the number of presynaptic input neurons divided by the number of starter neurons in RSCg. The individual circle represents one case (n = 5). Data are measured from M2-projecting RSCg neurons (N = 5 cases) and AD-projecting RSCg neurons (N = 5 cases) and are presented as the mean ± s.e.m. F, G Example of input- mapped local RSC subregions along the longitudinal axis. AP number indicates the distance relative to bregma. G represents magnified views corresponding to the panels in (F), indicating that the intrinsic connections vary across different layers along the anterior/posterior axis. H–L follow the same order as (A)–(E) and reveal RSC subregions inputs to AD-projecting RSCg neurons.

Journal: Molecular psychiatry

Article Title: Projection-specific circuits of retrosplenial cortex with differential contributions to spatial cognition.

doi: 10.1038/s41380-024-02819-8

Figure Lengend Snippet: Fig. 2 Monosynaptic rabies viral tracing reveals RSC intrinsic inputs to M2-projecting and AD-projecting RSCg neurons in C57BL/6 mice. A Schematic of monosynaptic retrograde rabies tracing (RV) of synaptic inputs to M2-projecting RSCg cells. The rAAV2-retro-Cre injection in M2 retrogradely drives Cre expression in M2-projecting RSC; then the Cre dependent AAV helper virus (AAV8-hSyn-DIO-TC66T-2A-EGFP-2A- OG) is injected into RSCg, followed by injection of an EnvA-pseudotyped SAD ΔG rabies virus three weeks later. B Immunostaining and microscopy images depict Cre positive neurons in the M2 injection site. rAAV2-retro-Cre labeled neurons are stained by a Cy5-conjugated secondary antibody and visualized as pink in the enlarged inset. C, D Coronal sections of injection in RSCg. Injection sites are shown in white boxes (C) with magnified images in (D). EGFP-labeled AAV-helper virus expressed in M2-projecting Cre+ neurons. Rabies is labeled by DsRed, DAPI is blue. White arrowheads indicate EGFP and DsRed double-labeled starter neurons. E Quantitative mapping of the connection strength of the intrinsic RSC subregion inputs to M2-projetcing RSCg neurons. Connection strength index (CSI) is calculated by the number of presynaptic input neurons divided by the number of starter neurons in RSCg. The individual circle represents one case (n = 5). Data are measured from M2-projecting RSCg neurons (N = 5 cases) and AD-projecting RSCg neurons (N = 5 cases) and are presented as the mean ± s.e.m. F, G Example of input- mapped local RSC subregions along the longitudinal axis. AP number indicates the distance relative to bregma. G represents magnified views corresponding to the panels in (F), indicating that the intrinsic connections vary across different layers along the anterior/posterior axis. H–L follow the same order as (A)–(E) and reveal RSC subregions inputs to AD-projecting RSCg neurons.

Article Snippet: A primary rabbit anti-Cre antibody (NOVUS, 1:500 dilution) was used, followed by a Cy5-conjugated donkey anti-rabbit secondary antibody (Jackson ImmunoResearch, West Grove, PA, USA, 1:200 dilution).

Techniques: Injection, Expressing, Virus, Immunostaining, Microscopy, Labeling, Staining